Journal: Oncology Letters

Article Title: A radioresistant fraction of acute promyelocytic leukemia cells exhibit CD38 cell-surface antigen and mRNA expression

doi: 10.3892/ol.2018.8099

Figure Lengend Snippet: Expression of CD38 mRNA in Res-HL60 cells. mRNA expression was quantified using reverse transcription-quantitative polymerase chain reaction. Values are presented as the means ± standard error of the mean of 4 separate experiments. *P<0.05 vs. Wt. CD, cluster of differentiation; Res, radioresistant; Wt, wild-type.

Article Snippet: Human APL wild-type (Wt) HL60 cells was purchased from RIKEN BioResource Center (Tsukuba, Japan).

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: A radioresistant fraction of acute promyelocytic leukemia cells exhibit CD38 cell-surface antigen and mRNA expression

doi: 10.3892/ol.2018.8099

Figure Lengend Snippet: Target cell fraction. (A) The CD38highCD45+ fraction of Res-HL60 cells, defined as the mean fluorescence intensity of >10 for CD38 expression using flow cytometry was isolated by fluorescence-activated cell sorting. The (B) cell-surface antigen and (C) quantified mRNA expression of the isolated CD38high cells were compared. The y-axis in (B) demonstrates the ratio of cell counts in each fluorescence channel. Data are presented as the mean ± standard error of the mean of 4 separate experiments. *P<0.05 vs. Res. CD, cluster of differentiation; Res, radioresistant; Wt, wild-type.

Article Snippet: Human APL wild-type (Wt) HL60 cells was purchased from RIKEN BioResource Center (Tsukuba, Japan).

Techniques: Fluorescence, Expressing, Flow Cytometry, Isolation, FACS

Journal: Oncology Letters

Article Title: A radioresistant fraction of acute promyelocytic leukemia cells exhibit CD38 cell-surface antigen and mRNA expression

doi: 10.3892/ol.2018.8099

Figure Lengend Snippet: Viable numbers of Wt-HL60 and Res-HL60 cells as assessed by the trypan blue method. The values are mean ± standard error of the mean of 4 separate experiments. *P<0.01. CD, cluster of differentiation; Res, radioresistant; Wt, wild-type.

Article Snippet: Human APL wild-type (Wt) HL60 cells was purchased from RIKEN BioResource Center (Tsukuba, Japan).

Techniques:

Journal: Oncology Letters

Article Title: A radioresistant fraction of acute promyelocytic leukemia cells exhibit CD38 cell-surface antigen and mRNA expression

doi: 10.3892/ol.2018.8099

Figure Lengend Snippet: Quantitative analysis of CD38, CD45 and CEBPA mRNA expression with or without exposure to 4 Gy X-irradiation at 24 h. Total RNA were isolated from each cell and the expression levels of mRNA were determined using strand-specific reverse transcription-quantitative polymerase chain reaction analysis. The values for mRNAs were normalized to the values of GAPDH mRNA, and the expression of each mRNA was compared as (each population)/(unexposed Wt-HL60 cells). Data are presented as the mean ± standard error of the mean of 4 separate experiments. *P<0.05 vs. unexposed Wt-HL60-CD38+/−cells. CD, cluster of differentiation; CEBPA, CCAAT/enhancer-binding protein α; Wt, wild-type; Res, radioresistant.

Article Snippet: Human APL wild-type (Wt) HL60 cells was purchased from RIKEN BioResource Center (Tsukuba, Japan).

Techniques: Expressing, Irradiation, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Binding Assay

Journal: Biomolecules

Article Title: Against Repurposing Methadone for Glioblastoma Therapy

doi: 10.3390/biom10060917

Figure Lengend Snippet: Tumoricidal methadone effects in preclinical studies as monotherapy or in combination with other anti-tumor therapies.

Article Snippet: CEM and HL-60 leukemia cells , 10–30 μM , subG 1 population PI-staining , apoptotic cell death IC 50 ~15 μM , [ ] .

Techniques: Activity Assay, Growth Assay, TUNEL Assay, Binding Assay, Staining, Western Blot, Viability Assay, Knockdown, Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Morphological changes of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p) after 24 h treatment.

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Apoptosis of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Cell cycle analysis of A549, A431, HL60, and IMR90 cell lines untreated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Cell Cycle Assay, Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Caspase-3 expression of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m), treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Percentage Cell Viability of A549, A431, HL60 and, IMR90 Cell-Lines Treated with Various Concentrations of SNP-B and SNP-C a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Control

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Apoptotic Study (i.e., Annexin V/PI Expression Study) of SNP-B and SNP-C against A549, A431, HL60, and IMR90 Cell-Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Cell Cycle Analysis of SNP-B and SNP-C against A549, A431 HL60, and IMR90 Cell Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Cell Cycle Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Caspase-3 Expression Study of SNP-B and SNP-C against A549, A431, HL60, and IMR90 Cell Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Fluorescence